Analysis of toxoplasma gondii proteins after Triton X-114 solubilization and hidropholic chromotograhy
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Spectroscopic Studies of Triton X-114 and Quercetin/Rutin Solutions
Flavonoids belong to a large group of natural polyphenol compounds and have a broad spectrum of pharmacological activities. Many natural flavonoids are characterized by extremely low solubility in aqueous media and body fluids. To improve the solubility of drugs, various methods were developed and are used in pharmaceutics: solubilization, preparation of solid disperse systems with soluble and ...
متن کاملSpectrophotometric determination of uranium in water samples after cloud point extraction using nonionic surfactant Triton X-114
A cloud point extraction process using the nonionic surfactant Triton X-114 to extracturanium from aqueous solutions was investigated. The method is based on the complexationreaction of uranium with Arsenazo III and micelle-mediated extraction of the complex. Theoptimal extraction and reaction conditions (e.g., pH, reagent concentration, effect of time) werestudied, and the analytical character...
متن کاملSolubilization of proteins from bovine brain coated vesicles by protein perturbants and Triton X-100
To identify integral and peripheral membrane proteins, highly purified coated vesicles from bovine brain were exposed to solutions of various pH, ionic strength, and concentrations of the nonionic detergent Triton X-100. At pH 10.0 or above most major proteins were liberated, but four minor polypeptides sedimented with the vesicles. From quantitative analysis of phospholipids in the pellet and ...
متن کاملPhase separation of integral membrane proteins in Triton X-114 solution.
A solution of the nonionic detergent Triton X-114 is homogeneous at 0 degrees C but separates in an aqueous phase and a detergent phase above 20 degrees C. The extent of this detergent phase separation increases with the temperature and is sensitive to the presence of other surfactants. The partition of proteins during phase separation in solutions of Triton X-114 is investigated. Hydrophilic p...
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SCOPE Investigations into the immunological response of proteins is often masked by lipopolysaccharide (LPS) contamination. We report an optimized Triton X-114 (TX-114) based LPS extraction method for β-lactoglobulin (BLG) and soy protein extract suitable for cell-based immunological assays. METHODS AND RESULTS Optimization of an existing TX-114 based phase LPS extraction method resulted in >...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 1988
ISSN: 0074-0276
DOI: 10.1590/s0074-02761988000400019